NANOFIL-100 - size: 100μL
NanoFil™ is a specially designed 10 µL syringe developed in response to customer requests for improved microinjection in mice and other small animals. It makes quantitative nanoliter injection much easier and more accurate than any other method currently in use.
Low Dead Volume
NanoFil's low dead volume eliminates the need for oil backfilling, a messy process which risks contamination of the injected sample. Injection is now simpler, and less messy, and there is no possibility of oil contamination in critical applications such as ophthalmology research (see the Retinal Pigment Epithelial (RPE) and Intra Ocular (IO) injection kits listed below).
Easily Switch Syringe Tip
When the inner tip diameter of a conventional syringe is reduced to less than 100 µm, it is very difficult to front fill the solution at a reasonable speed. NanoFil solves this problem by using a tip coupling mechanism that makes it possible to change the syringe tip during the experiment. Simply load the sample using a larger tip, such as the 26 gauge needle provided with the syringe, and then replace it with a micro tip for sample injection. On a conventional 10 µL syringe, a solid ring or bushing is permanently bonded to the tubing. Replacing the tip in the middle of the experiment is not practical. With NanoFil, tips can be exchanged by a simple twist of the brass lock, gently pulling out the tip, and replacing with the desired new tip.
Holds Metal Tips and Quartz Tubing
To secure the tip, NanoFil uses an olive-shaped silicon gasket that is similar to, but much sturdier than, some of the microelectrode holders used for electrophysiology recording. The silicone gasket makes it possible to hold not only metal needles but also Silflex tubing. Many types of tubing can be easily connected to the syringe as long as the outer diameter (OD) is close to, but not more than, the barrel inner diameter (ID) of 460 µm. Flexible quartz capillaries used in Gas Chromotography (GC) and Capillary Electrophoresis (CE) can also be easily coupled to the syringe.
Variety of Tips
Specially designed needles as small as 36 gauge (110 µm OD) are offered in both blunt and beveled styles. Our studies have shown that these needles will cause less trauma to the tissue. NanoFil has a unique coupling mechanism that allows many different forms of small tubing and tips to be coupled with the syringe barrel.
In the video below, you can see how to front fill an Nanofil syringe. See the full-size video.
NanoFil for Microinjection
The NanoFil-100 is a 100µL syringe with a small dead volume.
The NanoFil syringe is a 10µL syringe. NanoFil is a specially designed syringe developed in response to customer requests for improved microinjection in mice and other small animals. It makes quantitative nanoliter injection much easier and more accurate than any other method currently in use.